Bioplastics

Biography

Biography: Anand Gadre

Abstract

Advanced biocompatible polymers  and co-polymers have been successfully implemented to generate electrospun nanofibers for variety of biological and biomedical applications. This work focused on generating a biopolymeric drug delivery system using (Poly-Lactic- co-Glycolic Acid (PLGA). Controlled drug elution targets a specific location to avoid overdosing and thereby reducing the side effects of the drug. In this study, we prepared a local anesthetic (Lidocaine) as  well as  an  antibiotic (Chloramphenicol) loaded PLGA nanofibers using electrospinning technique   and   evaluated   the   drug release activity at a stable rate. Electrospinning is performed by applying a large positive voltage (several kV) to a metal syringe tip, which dispenses a polymer solution. The potential field draws a fiber from the end of the tip and deposits a randomly oriented and non- woven mat of polymeric fiber on a grounded metal collector plate. Depending on spinning parameters, fibers ranging from as small as 100 nm to as large as several micrometers can be achieved. We can alter the fiber diameter to control the rate of delivery. Fibers of lager diameter will have smaller surface area to volume ratios and should therefore release drug molecules compounds at a slower rate, but for a longer   period   of   time.  Conversely, smaller diameter fibers could release drug molecules at a faster rate, which could be beneficial for initial treatment.
Structural and optical properties of such drug loaded fibers confirmed the presence of drug molecules. Systematic drug elution experiments using UV- spectrophotometry were performed to observe possible indication in the absorbance change as the drug elutes from the nanofibers over the period of time. Drug loaded biopolymeric nanofibers also showed limited cytotoxicity on fibroblasts with viability greater than 96%. Such drug-loaded biopolymeric nanofibers can be applied via different routes, such as implantation, injection, and/or oral administration for a wide range of medical treatments.